Gel/PCR DNA Fragments Extraction Kit,100 Rxn, Cat.: ADFH100
Technical Specs: Sample: up to 300 mg of agarose gel up to 100 μl of PCR products Recovery: up to 95% Format: spin column Operation time: 20 minutes Elution volume: 20-50 μl
The Gel/ PCR Fragment Extraction Kit was designed to recover or concéntrate DNA fragments (100 bp to 10 Kb) from agarose gel, PCR, or other enzymatic reactions. Contaminants are removed with a Wash Buffer (containing ethanol) salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction alcohol precipitation. Typically, recoveries are up 90% for Gel Extraction and up to 95% for PCR Clean up. The entire procedure can be completed in 20 minutes and the eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation. or alcohol precipitation. Typically, recoveries are up 90% for Gel Extraction and up to 95% for PCR Clean up. The entire procedure can be completed in 20 minutes and the eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.
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