HOT FIREPol® EvaGreen® HRM Mix Without ROX. 1 ML | 250 rxn. Cat.: 083100001
high resolution melt (HRM)
highly sensitive mix for accurate SNP detection
sensitive EvaGreen® dye for reliable HRM performance
reaction set-up without ice
5x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup
083100001
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high resolution melt (HRM)
highly sensitive mix for accurate SNP detection
sensitive EvaGreen® dye for reliable HRM performance
reaction set-up without ice