HOT FIREPol® is a chemically modified FIREPol® DNA Polymerase.

HOT FIREPol® is inactive at room temperature and is activated by a 15 min incubation step at 95°C. This enables hot-start PCR and minimizes mispriming and extension from non-specifically annealed primers and primer-dimers. Recommended for routine applications (fragment up to 5 kb).

Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.

The fidelity of HOT FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide app. 2.5 x10-5).