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iPLEXSTR is a short tandem repeat (STR) multiplex PCR kit that includes 13 CODIS STR loci (D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA), 5 European standard STR loci (D1S1656, D2S441, D10S1248, D12S391, and SE33) and gender determination locus Amelogenin. iPLEXSTR PCR primers have been designed to generate products between 75 and 420 bp. labelled with blue, green, yellow and red fluorescent dyes. Sizing standards provided with the kit are labelled with orange dye and contain 23 DNA fragments of 70, 80, 90, 100, 120, 140, 160, 180, 200, 220, 230, 240, 260, 280, 300, 320, 340, 360, 380, 400, 420, 440 and 450 bp.
All components of iPLEXSTR kit, except for activator solution, are provided in lyophilised format, which allows storage of the kit at room temperature for one year without loss of sensitivity. Lyophilised PCR master mix includes primers and hot start polymerase and is aliquoted into 0.2 mL PCR-reaction tubes. The reaction components are activated by addition of 5 μL of activator solution to each tube. For final PCR volume of 25 μL, up to 20 μL of DNA solution can be added per reaction. Full STR-profiles can be generated from as little as 100 pg of non-degraded genomic DNA in a full size (25 μL) PCR reaction. iPLEXSTR kit is very robust and can generate excellent results from 6 μL PCR reactions. Full STR-profiles can be generated from as little as 25 pg of non-degraded genomic DNA in a 6 μL PCR reaction.
iPLEXSTR allows direct amplification from unpurified TE-Chelex extracts of buccal swabs. iPLEXSTR kit contains an Internal Control that can help assess the presence of PCR inhibitors: the lyophilized master mix contains a set of blue dye labelled primers which will amplify a high molecular weight product (400 - 440 bp) whose presence and peak height correlate with the purity of the DNA extract.
iPLEXSTR kit is compatible with ABI PRISM® 310, 3100, 3130 and 3500 Genetic Analyzers.