5x HOT FIREPol® EvaGreen®qPCR Mix Plus (ROX)(1 mL), 250 rx View larger

5x HOT FIREPol® EvaGreen®qPCR Mix Plus (ROX)(1 mL), 250 rx

08-24-00001

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HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye. The user simply needs to add water, template and primers. 


HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup