5x HOT FIREPol® EvaGreen®HRM Mix (ROX) 1mL

5x HOT FIREPol^® EvaGreen^® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen^® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂, EvaGreen^® dye and ROX dye according to system requirements. The user simply needs to add water, template and primers.

HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.