Easy visualization of the real time progress of your gel electrophoresis
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6X Loading dye is most commonly used in order to monitor the progress of an electrophoresis run. It is combined with DNA samples in order to ensure that samples do not diffuse away when being loaded and also to provide easy visualization during loading. abm’s DNA Loading Green dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.
Protocol: Mix at least 1ul of the 6X DNA Loading Dye to every 5ul of the total sample volume before loading in the gel.