Specifications Sample: up to 200 µl plasma, serum, body fluid or the supernatant of virally infected cell cultures Format: spin column Yield: up to 50 ug Operation Time: < 20 minutes Applications: Real-time PCR/RT-PCR, DNA Sequencing, PCR, and other enzymatic reactions. Storage: room temperature for at least 9 months
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The Viral Nucleic Acid Extraction Kit II was designed specifically for viral DNA/RNA purification from cell-free samples such as serum, plasma, body fluids and the supernatant of virally infected cell cultures. Contaminants, such as salts, metabolites and soluble macromolecular cellular components are removed in the wash step. The nucleic acid is eluted in the RNase-free water ready for use in subsequent reactions, including Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. This protocol is recommended for parallel purification of viral DNA including HBV and CMV and viral RNA including HCV, HIV, and HTLV.