Specifications Sample: up to 200 μl of blood, buffy coat), up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue Format: spin column Yield: up to 50 ug RNA Operation Time: 10 minutes Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting Storage: Room temperature fo
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The TriRNA Pure Kit is a spin column system for purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in TRIzol® Reagent, GENEzol™ Reagent or otherphenol, guanidine isothiocyanate reagents. Chloroform phase separation or isopropanol RNA precipitation is not required. Dnase I is included with this RNA kit for DNA sensitive downstream applications. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of downstream applications.